Trial no.:	PACTR202405535547940	Date of Approval:	21/05/2024
Trial Status:	Retrospective registration - This trial was registered after enrolment of the first participant

TRIAL DESCRIPTION

Public title

Investigating the impact of two dynamic stretching techniques during warm-up on oxidative stress biomarkers, both enzymatic and non-enzymatic aspects, following a supramaximal exercise Intel 72 hours

Official scientific title

Evaluation of the effects of two active stretching techniques performed during warm-up on markers of oxidative stress following anaerobic exercise in active subjects

Brief summary describing the background and objectives of the trial

This study explored the intricate relationship between oxidative stress , physical activity and stretching; We investigated various factors influencing reactive oxygen species (ROS) production. Our study underscored the pivotal role of stretching technique in activating adaptive cellular responses, yet caution against the potential for excessive muscle exertion provost by supramaximal exercise to induce injury. Notably, warm-up routines emerge as a crucial strategy for mitigating oxidative stress and injury risk, with a 15-minute warm-up showed to reduce muscle damage and elevate antioxidant levels. Methods 23 young athletes made up of individuals with similar levels, all participating in at least 5 hours of weekly physical activity. who underwent the VMA vitesse max aerobic test following a standardized warm-up were inclued either active-dynamic stretching or active myotendinous stretching or no stretching (rest) Several blood samples were taken: at rest (PR), after warm-up (PWU) and immediately after stopping each event (P0), at 10 min (P10), at 20 min (P20), then at 24h (P24), and at 72h (P72) of recovery. Parameters measured were MDA, PC, SOD, GR, GPX, AU, FRAP and SAT. The objectif of this study is to investigate and explore the impact of two different dynamic stretch (active-dynamic ADS, active myotendinous AMTS) with warm-up as it was not well introduced and how they can control the response of oxidative stress biomarkers post supramaximal exercise up to 72h to overview. Keyword: reactive oxygen species, ROS, oxidative stress, dynamic stretching, supramaximal exercise, antioxidant capacity, muscle damage.

Type of trial

Non-Randomised

Acronym (If the trial has an acronym then please provide)

Disease(s) or condition(s) being studied

oxidative stress

Sub-Disease(s) or condition(s) being studied

Purpose of the trial

Prevention

Anticipated trial start date

17/12/2023

Actual trial start date

17/12/2023

Anticipated date of last follow up

17/12/2023

Actual Last follow-up date

17/12/2023

Anticipated target sample size (number of participants)

23

Actual target sample size (number of participants)

23

Recruitment status

Completed

Publication URL

Secondary Ids	Issuing authority/Trial register

STUDY DESIGN
Intervention assignment	Allocation to intervention	If randomised, describe how the allocation sequence was generated	Describe how the allocation sequence/code was concealed from the person allocating the participants to the intervention arms	Masking	If masking / blinding was used
Crossover: all participants receive all interventions in different sequence during study	Non-randomised			Open-label(Masking Not Used)

INTERVENTIONS
Intervention type	Intervention name	Dose	Duration	Intervention description	Group size	Nature of control
Control Group	control groupe	Warm-up Jogging 10min Rest 2min Specific movement 5min	17min + 7min rest	Warm-up Jogging 10min Rest 2min Specific movement 5min Heels to buttocks Hamstrings (6s effort /6s rest) Jumps on toes in a bipodal Triceps surae (6s effort /6s rest) Squats 90° hip and knee flexion Quadriceps (6s effort /6s rest)	23	Active-Treatment of Control Group
Experimental Group	exprimental groupe 1	Active-dynamic stretching (Stretch/ Contraction/ release and dynamic phase)	warm-up 17min +2min rest + active-dynamic stretching (4min30)	Active-dynamic stretching (Stretch/ Contraction/ release and dynamic phase) Dynamic phase 12s Heels to buttocks Jumps on toes in a bipodal Squats 90° hip and knee flexion Contraction 4s Stretch 4s Release 2 times for each muscle (Rest 5s in between) * 2 limbs	23
Experimental Group	experimental group 2	Active myotendinous stretching	warm-up 17min +2min rest + active myotendinous stretching (5min)	Active myotendinous stretching Eccentric movement 8s Isometric (static) contraction 8s Stretching 8s 2 times for each muscle Hamstrings/triceps surae /quadriceps For the 2 lower limbs With alternation	23

ELIGIBILITY CRITERIA
List inclusion criteria	List exclusion criteria	Age Category	Minimum age	Maximum age	Gender
healthy diet did not taking creatine regularly Consuming dietary or supplemental products that contained antioxidants and polyphenols was prohibited in the 3 months leading up to the study	no injuries no illnesses did not smoke did not suffered from pathological sleep disorders did not consumed alcohol	Adult: 19 Year-44 Year	20 Year(s)	20 Year(s)	Male

ETHICS APPROVAL

Has the study received appropriate ethics committee approval

Date the study will be submitted for approval

Date of approval

Name of the ethics committee

Yes

08/12/2023

Individual Protection Committee South

Ethics Committee Address
Street address	City	Postal code	Country
manzel chaker	sfax	3029	Tunisia

OUTCOMES
Type of outcome	Outcome	Timepoint(s) at which outcome measured
Primary Outcome	MDA Malondialdehyde PC Protein carbonyl SOD Superoxide dismutase GPx Glutathione peroxidase GR Glutathione reductase FRAP Ferric Reducing Ability of Plasma SAT Total antioxidant status	rest/ post intervention/ immediately post maximal exercise/ after10min/ after 20min/ after 24hours/ after 72 hours
Secondary Outcome	AU Uric acid	rest/ post intervention/ immediately post maximal exercise/ after10min/ after 20min/ after 24hours/ after 72 hours

RECRUITMENT CENTRES
Name of recruitment centre	Street address	City	Postal code	Country
the superior Institut of sport and physical education	route matar	sfax		Tunisia

FUNDING SOURCES
Name of source	Street address	City	Postal code	Country
self funded	palmariome 1 bloc b n32	sfax	3029	Tunisia

SPONSORS
Sponsor level	Name	Street address	City	Postal code	Country	Nature of sponsor
Primary Sponsor	Laboratory EM2S	route matar	sfax	3029	Tunisia	Research Laboratory

COLLABORATORS
Name	Street address	City	Postal code	Country

CONTACT PEOPLE
Role	Name	Email	Phone	Street address
Principal Investigator	mejda alia	dalizar_mejda@hotmail.fr	+21159712	palmariome 1 bloc b n32
City	Postal code	Country	Position/Affiliation
sfax	3029	Tunisia	LR19JS01
Role	Name	Email	Phone	Street address
Public Enquiries	mejda alila	dalizar_mejda@hotmail.fr	+21621159712	palamriome 1 bloc b N32
City	Postal code	Country	Position/Affiliation
sfax	3029	Tunisia	LR19JS01
Role	Name	Email	Phone	Street address
Scientific Enquiries	mejda alila	dalizar_mejda@hotmail.fr	+21621159712	palmariome 1 bloc b n32
City	Postal code	Country	Position/Affiliation
sfax	3029	Tunisia	LR19JS01

REPORTING
Share IPD	Description	Additional Document Types	Sharing Time Frame	Key Access Criteria
Yes	Individual participant data that underlie the results reported in this article, after deidentification (text, tables, figures, and appendices)	Study Protocol	6 months following papers publication	Researchers who provide a methodologically sound proposal
URL	Results Available	Results Summary	Result Posting Date	First Journal Publication Date
	No
Result Upload 1:	Result Upload 2:	Result Upload 3:	Result Upload 4:	Result Upload 5:

Result URL Hyperlinks	Link To Protocol
Result URL Hyperlinks

Changes to trial information

Pan African Clinical Trials Registry